Antiinflammatory Profile of Alnus nepalensis Leaves

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Antiinflammatory Profile of Alnus nepalensis Leaves

Antiinflammatory profile of Alnus nepalensis leaves extracts Invitro and invivo study

Archana Saxena1 Deepti Yadav2 Anant kumar1 Madan MGupta2 Dnyaneshwar UBawankule1



ABSTRACT

Ethnopharmacological relevance Alnus nepalensis has been used in the traditional medicine to treat the inflammatory conditions in India Korea and China but it lacks proper pharmacological intervention

Aim of the study To evaluate the invitro and invivo antiinflammatory profile of A nepalensis leaves extracts to provide experimental evidence for its traditional use

Materials and methods Methnolic extract ANM hexane fraction ANH chloroform fraction ANC and butanol fraction ANB were obtained from air dried leaves of A nepalensis These were tested invitro against lipopolysaccharide LPSinduced inflammation in macophage cells by quantifying the production of proinflammatory cytokine ANB a most active fraction was further validated for invivo antiinflammatory study The quality and the reproducibility of ANB was ensured by highperformance liquid chromatography fingerprint

Results Invitro results revealed that ANB exhibited significant antiinflammatory effect Invivo study further confirmed its antiinflammatory property by significant P005 inhibition of carrageenaninduced paw edema in dose dependant manner The HPLC analysis of ANB showed the presence of diarylheptanoids

Conclusion These results revealed that ANB a butanol fraction of Anepalensis possess the significant antiinflammatory activity which supporting the traditional usage of the plant to treat inflammatory diseases

Key words Alnus nepalensis Diarylheptanoids Inflammation Macrophages Rats
Introduction

Alnus nepalensis belongs to the family Betulaceae an alder species and openpollinated forest tree found in the hilly regions Chauhan and Misra 2002 Almost all plants of genus Alnus have been used in the traditional medicine in India Changkija 1999 Korea Lee 1996 and China Vo1997 to treat inflammatory diseases diarrhea dysentery and fever Platyphyllenone alusenone hirustenone and hirsutanonol are the important pharmacological active diarylheptanoids isolated from Alnus species Lee et al 2000 Hirustenone and hirsutanonol show antiinfluenzal Tung et al 2010 antioxidant Kuroyanagi et al 2005 cytotoxic Choi et al 2008 hepatoprotective Park et al 2010 antiinflammatory activity Kim et al 2005 Furthermore hirustenone has been reported to prevent cytokine and chemokinemediated immune cell function and inflammatory reaction and was found to be an attractive starting point for the development of a topical drug for T cellbased antiatopic dermatitis due to its calcineurin inhibitory effects Joo et al 2009 Alusenone and platyphyllenone showed significant antioxidant and hepatoprotective effects Tung et al 2010 Recently our group has reported that diarylheptanoids isolated from A nepalensis leaves exhibiting invitro and invivo Antifilarial activity Yadav et al2013

Inflammation is the first response of the immune system to infection injury or irritation and macrophages play a crucial role during the inflammatory process Lalenti et al1993 Chronic inflammation is a prolonged dysregulated and maladaptive response that involves active inflammation tissue destruction and attempts at tissue repair Such persistent inflammation is associated with many chronic human diseases including allergy atherosclerosis gastroenteritis arthritis autoimmune diseases etc To overcome the challenges of inflammatory disorders several classes of antiinflammatory drugs have been used Despite considerable progress in the treatment of various inflammatory diseases and disorders using modern therapeutic agents search for newer drugs continues because the existing synthetic drugs have several side effects The current trend in research is the investigation of medicines of plant origin because of their affordability and accessibility with minimal side effects Calixto 2005 Considering the traditional use of A nepalensis

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