Determination of Stomatal Index

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Determination of Stomatal Index

Keywords stomatal index calculation stomatal index procedure

The Plant material of Viscum capitellatum Smith parasitism on Dendrophthoe falcata which is itself parasitic on M indica was collected from Amba Ghat Kolhapur Western Ghat region of Maharashtra from India in November 2009 The collection are lies Latitude 16o 58 059N and Longitude 73 48 3661E at altitude 1100m The plant specimen Voucher no 550 was authenticated by Dr Vinay Raole Reader Department of Botany MS University Baroda India

Pharmacognostical Study

Macroscopical Study68

It includes the shape size colour texture surface and odour of the drug in crude or powered form and often sufficient to enable to identify the whole drugs

Microscopical Study

Histochemistry

It gives the idea about the colour reaction of specific chemical reagent towards plant tissues 68 Microscopical images are given in Figure no 2

Quantitative Microscopy 6669

Transverse sections of scale and stems were obtained by means of a microtome and stained with different staining reagents as per standard procedures 66 7071 All observations were performed using Motic Digital Photomicroscope

Histological study of leaves and stem were performed by reported method 69 Leaves were boiled in a 5 aqueous solution of NaOH for 5 min while stems were boiled with 10 aqueous solution of NaOH for 10 min After cooling and washing with water pieces were treated with a 25 aqueous solution of chromic acid for 30 min at room temperature Washed pieces of both leaf and stem were pressed in between two slides and slides coves

Determination of Stomatal Number

The average number of stomata per square millimeter of epidermis is termed the stomatal number

Determination of Stomatal Index

The percentage proportional to the ultimate divisions of the epidermis of a leaf which has been converted into stomata is termed the stomatal index

SI S 100

E S

Where SI Stomatal index S number of stomata per unit area and E number of ordinary epidermal cells in the same unit area

Procedure 68

Pieces of leaf between margin or midrib was cleared and mounted and the lower surface examined by means of a microscope with a 4mm objective and an eyepiece containing a 5mm square micrometer disc Counts were made of the numbers of the epidermal cells and of stomata within a square grid a cell being counted if at least half of its area lies within the grid The stomata index was determined for both leaf surfaces Results pertaining to quantitative microscopical study are given in table no 8

Analytical Study

Ash Value68 72

11 Total ash

Total ash gives the idea about the residue obtained after ignition It consist of physiological ash obtain by ignition of plant tissues and non physiological ash obtain by ignition of extraneous matter adhering to the surface of Plant 2 gm of accurately weighed air dried powdered drug was taken in silica crucible This silica crucible with drug material was kept in muffle furnace and ignited at temperature 4500C The material was heated till the white coloured ash and constant weight is obtained The procedure was performed in triplicate Result is given in table No 9

The total ash was calculated by subtracting the weight of crucible with ash of drug after ignition from weight of crucible with drug powder before ignition Percentage of total ash was calculated with reference to airdried drug

Acid insoluble ash

Acid insoluble ash gives the idea about the presence of inorganic material such as calcium oxalate present in plant material

The ash obtained in the total ash method was boiled with 25 ml of 2N hydrochloric acid for 5 min Insoluble matter was collected on ash less filter paper Whatman paper and washed with hot water The material retained on filter paper and along with filter paper was further ignited and weighed Percentage of acid insoluble ash was calculated with reference to air dried material Result is given in table No 9

Water soluble

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