Diversity of Fungal Endophytes in Cymbopogon Caesius

  • Home
  • /
  • India
  • /
  • Diversity of Fungal Endophytes in Cymbopogon Caesius

Diversity of Fungal Endophytes in Cymbopogon Caesius

Diversity of Fungal Endophytes in Cymbopogon caesius Hook Arn Stapf of Kemmannugundi Regions of Karnataka India

Avinash KS and YL Krishnamurthy



Abstract

Cymbopogon grass produces many bioactive compounds and used for many medicinal purposes In this study healthy leaf stem and root segments were cultured on media like Potato dextrose agar and Malt extract agar medium and pure cultured Endophytic fungi were isolated from 1200 samples of Cymbopogon caesius grass collected from Kemmannugundi regions of Karnataka Overall 56 of colonisation rate from surface sterilised tissues were recorded About 30 different fungal species were isolated and 12 were identified and 18 were grouped as morph taxa Less isolates were recovered from leaf segments and more isolates were recovered from root segments Curvularia sp and Fusarium sp were frequently isolated endophytes with the high colonisation rate

Key Words Endophytes Cymbopogon caesius Grass Curvularia Fusarium

1 Introduction

Endophytes are microorganisms which live inside the host without causing any negative effect by their presence The plant and endophytic fungi show symbiotic association Plant protects and feeds the endophyte which in return produces bioactive secondary metabolites to enhance the growth and competitiveness of the host in nature Carrol 1988 and Hong Lu et al 2000 Endophytes are widely investigated for their bioactive metabolites and have proven to produce potential compounds which are applicable in medicine Clovis and Ewald 2012 The compounds usually isolated from medicinal plants may not be plant metabolites they may be from fungal endophytes which reside in the host plant Endophytes of tropical plants are among the groups of fungi that have been studied to arrive at the predicted figure of 15 million Hawksworth 1991 Subramanian et al 2003

Cymbopogon species are traditional medicinal grasses well recognised for its aromatic oil Cymbopogon caesius grass was wide spread in areas of Kemmannugundi of Chikmagalur Dist Karnataka Despite the wide distribution of Cymbopogon grass along the central Western Ghats of Karnataka only limited work has been done for their association with fungal endophytes Even though the oil extracted from the grass has been used in medicine currently there is no enough information regarding the diversity of the fungal endophytes with the species We have selected Cymbopogon caesius Hook Arn Stapf for the study of diversity of the fungal endophytes Fungi were isolated from leaves stem and root followed by the surface sterilisation process

The objectives of the study were to isolate and identify the fungal endophytes from different parts of Cymbopogon caeceous grass of Kemmannugundi regions of Karnataka

2 Meterials and Methods

21 Sampling

C caeseus root stem and leaf samples was collected from Kemmannugundi 132826 N 754450 E 4275ft Chikmagalur District Roots were collected with a ball of soil so that to avoid damage to the roots Twenty healthy looking culms of grass were collected and transported in a clean polythene bag in closed condition and processed within 24 hour of collection

22 Isolation of fungi

The collected samples were washed thoroughly by running tap water to clean soil from roots and were cut into small pieces The cut segments were surface sterilised using 70 ethanol followed by immersion in 3 Sodium hypochlorite for 4 minutes and again washed twice with distilled water Maheshwari and Rajagopal 2013 The efficacy of the surface sterilisation was confirmed by pressing the sterilised segments onto the surface of the medium the surface sterilised small pieces were cut into 0305cm segments Chuyang et al 2001 Four hundred segments were inoculated into potato dextrose agar medium which is supplemented with 100mg of Amoxillin to inhibit the growth of bacteria The inoculated petri plates were wrapped with petriseal and incubated at 27 1 degree centigrade The plates were observed daily after 3 days of in

* If you are the original writer of this essay and no longer wish to have the essay published on the WetPapers website then please email us at wet papers 1 @ gmail dot com for removal request.

© 2018 WetPapers. All rights reserved. Privacy Policy Terms of Service Disclaimer Copyright